Cytosolic protein concentration is the primary volume signal in dog red cells.
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منابع مشابه
Cytosolic protein concentration is the primary volume signal in dog red cells
It is not known whether the activation of Na/H exchange by shrinkage in dog red cells is due to the packing of cell contents or a change in cell configuration. To make this distinction we prepared resealed ghosts that resembled intact cells in hemoglobin concentration and surface area, but had one-third their volume. A shrinkage-induced, amiloride-sensitive Na flux in the ghosts was activated a...
متن کاملCytosolic protein concentration is the primary volume signal for swelling-induced [K-Cl] cotransport in dog red cells
Chloride-dependent K transport ([K-Cl] cotransport) in dog red cells is activated by cell swelling. Whether the volume signal is generated by a change in cell configuration or by the dilution of some cytosolic constituent is not known. To differentiate between these two alternatives we prepared resealed ghosts that, compared with intact red cells, had the same surface area and similar hemoglobi...
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Red blood cells from mature dogs contain less Na and more K than would be the case if they were in Donnan equilibrium with plasma. They have no ouabain-sensitive Na pump, and their membranes are deficient in Na, K-ATPase. Experiments are reported in which dog red cells were first loaded with supranormal quantities of Na and water and then reinjected into the dog. Over the course of 26-40 h the ...
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Studies have been made on the cation transport system of the dog red cell, a system of particular interest because it has been shown that there is a marked dependence of cation fluxes on the cell volume. We have found that a 10% decrease in cell volume causes a large increase in 1 hr uptake of (24)Na as well as a considerable inhibition of (42)K uptake. This effect cannot be produced by a diffe...
متن کاملThe Protein Concentration in the Proximal Tubule of the Dog.
The mammalian proximal tubular fluid is generally considered to contain a low but definite protein concentration (1-3). The degree of glomerular capillary membrane permeability has been considered to range from that of a perfect ultrafilter to one yielding a protein concentration of 30 mg per 100 ml or more in the immediate filtrate. The most obvious evidence that protein is present in the glom...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1991
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.98.5.881